Internal Membranous Structure in Bacillus Macerans Spores.

Adenine and 2,6-diaminopurine as germinants for Bacillus macerans spores.

Germination requirements of Bacillus macerans spores.

2-Phenylacetamide is an effective germinant for spores of five strains of Bacillus macerans, particularly in the presence of fructose. Benzyl penicillin, the phenyl acetamide derivative of penicillin, and phenylacetic acid are also good germinants. l-Asparagine is an excellent germinant for four strains. alpha-Amino-butyric acid is moderately effective. Pyridoxine, pyridoxal, adenine, and 2,6-d...

Effect of subtilin and nisin on the spores of Bacillus coagulans.

The early literature on the effect of the polypeptide antibiotics subtilin and nisin on bacterial spores has been reviewed by Campbell and O'Brien (1955). Most of the early reports have indicated that subtilin acts as a sporostatic agent. Other reports have shown, however, that the spores of several bacterial species will germinate in the presence of subtilin and are subsequently killed by the ...

Rapid in situ hybridization technique using 16S rRNA segments for detecting and differentiating the closely related gram-positive organisms Bacillus polymyxa and Bacillus macerans.

A rapid, sensitive, inexpensive in situ hybridization technique, using 30-mer 16S rRNA probes, can specifically differentiate two closely related Bacillus spp., B. polymyxa and B. macerans. The 16S rRNA probes were labeled with a rhodamine derivative (Texas Red), and quantitative fluorescence measurements were made on individual bacterial cells. The microscopic fields analyzed were selected by ...

Catabolism of protocatechuate by Bacillus macerans.

An aerobic endospore-forming bacterium, tentatively identified as a strain (JJ-lb) of Bacillus macerans, was isolated by enrichment on 4-hydroxybenzoate (4HBA), using as an inoculum soil taken from a 50 degrees C Iadho hot spring. Enzymatic analyses of cells grown on succinate and 4HBA indicated that strain JJ-1b degrades 4HBA by way of the novel protocatechuate (PCA) 2,3-dioxygenase pathway. P...